Insertion junction: LMJ.RY0402.150320_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.150320
Chromosome:chromosome 16
Location:1512213
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g653150 CPR3 (1 of 2) PF01841 - Transglutaminase-like superfamily (Transglut_core); Cysteine protease, transglutaminase-like 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATAGCGACAGGAAAATAATCAAGTATGAA
Internal bar code:GCGGGAGAGGTCGGTCGATCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:355
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:97
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCGAGGTCAAGGAATGTC
Suggested primer 2:TGACAGGAAGAAGATTGGGG