| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150367 |
| Chromosome: | chromosome 1 |
| Location: | 1696202 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008950 | (1 of 1) IPR024930 - Skp domain | 5'UTR|outside_mRNA | |
| Cre01.g008976 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGCTTGCCAAGTGGCCTTCAGCTGCAC |
| Internal bar code: | GCTTAGGGAGCGAACGGTGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 825 |
| LEAP-Seq percent confirming: | 99.1919 |
| LEAP-Seq n confirming: | 1473 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTGAAAGAACGCAACAC |
| Suggested primer 2: | AGCTCCGACAGGAAACTCAA |