| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150378 |
| Chromosome: | chromosome 12 |
| Location: | 6818070 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561450 | (1 of 1) PTHR11228//PTHR11228:SF23 - RADICAL SAM DOMAIN PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAGTCATAACCAGTTCGAACGCTCCATC |
| Internal bar code: | ACCGCCTACATGTGTTCGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 99.5653 |
| LEAP-Seq n confirming: | 3665 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTTACGAACGATGGGTGC |
| Suggested primer 2: | CAAGTACCTGCAGCACCAAA |