| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150482 |
| Chromosome: | chromosome_9 |
| Location: | 2557114 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390750 | ELG24 | Exostosin-like glycosyltransferase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTACTATGGGCCCCCTTTCCAAACAGCAA |
| Internal bar code: | ATTTTTTCCGTCTATATAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 982 |
| LEAP-Seq percent confirming: | 99.8538 |
| LEAP-Seq n confirming: | 1366 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAAGAAGTCGGCCTCCTC |
| Suggested primer 2: | AATAATGGACTTGGGAGGGG |