| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150511 |
| Chromosome: | chromosome 12 |
| Location: | 4351142 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520173 | (1 of 1) PTHR10887//PTHR10887:SF340 - DNA2/NAM7 HELICASE FAMILY // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGGCGCTGCTGGTGGCGGGGGAGGG |
| Internal bar code: | AGTCGTTGGTGCTTTGCGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 99.7802 |
| LEAP-Seq n confirming: | 454 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACCGCCATAGATACG |
| Suggested primer 2: | TGGTGCTAGTGGTTGAGGTG |