| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150545 |
| Chromosome: | chromosome 13 |
| Location: | 3896123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590450 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCAGGCGCGTCGCTGCATGTACTGTGG |
| Internal bar code: | GATGATGGGTTCTACCGGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 242 |
| LEAP-Seq percent confirming: | 99.7406 |
| LEAP-Seq n confirming: | 3076 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTCCTGGAGCTATGGCA |
| Suggested primer 2: | TCGTCTACACAGCTCATCCG |