| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150552 |
| Chromosome: | chromosome 4 |
| Location: | 2769669 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223700 | (1 of 2) K07735 - putative transcriptional regulator (algH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATCAGACACGGCATGAGGTGTGACGGGG |
| Internal bar code: | AGTTTAATTGCGAGTGAGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 98.9848 |
| LEAP-Seq n confirming: | 195 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACGGAAGAACACACCCT |
| Suggested primer 2: | CTCCAAACCTGCACTTGTGA |