| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150560 |
| Chromosome: | chromosome 9 |
| Location: | 2184510 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393150 | FOX1,FLP | Multicopper ferroxidase; (1 of 2) K14735 - hephaestin (HEPH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGTGTTGAGCCGCGAACTTCGGTTGT |
| Internal bar code: | CCGGGTGGGCCTGACGCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 99.8131 |
| LEAP-Seq n confirming: | 2670 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTACACGACTTCAGGGCA |
| Suggested primer 2: | CGGTTATGTTGCACTGTTGG |