| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150568 |
| Chromosome: | chromosome 12 |
| Location: | 5458715 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACTCCCCGCGCCCTTAGGACTGTGCCT |
| Internal bar code: | TGGGATCGCTTTGTTTGCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 99.515 |
| LEAP-Seq n confirming: | 1231 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCAAGACCGTGCTCAAG |
| Suggested primer 2: | GGTTCTGCTGTCCGCTAGAC |