| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150578 |
| Chromosome: | chromosome 1 |
| Location: | 4448206 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030200 | PP2C4,PPP3,FAP6 | Flagellar Associated Protein 6; (1 of 1) PTHR13832:SF273 - PROTEIN TAP-1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCTTGCATTCGGAATCCACCTCAGCAA |
| Internal bar code: | GCGTTTAGATCTCTTTGCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 222 |
| LEAP-Seq percent confirming: | 99.6016 |
| LEAP-Seq n confirming: | 5000 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGCTACCTGGCAAACAT |
| Suggested primer 2: | AGGTCCACCTTCACCTCCTT |