Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.150578 |
Chromosome: | chromosome_1 |
Location: | 4448206 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g030200 | PP2C4,PPP3 | Phosphoprotein phosphatase 2C delta | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCGTCTTGCATTCGGAATCCACCTCAGCAA |
Internal bar code: | GCGTTTAGATCTCTTTGCAGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 222 |
LEAP-Seq percent confirming: | 99.6016 |
LEAP-Seq n confirming: | 5000 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGGCTACCTGGCAAACAT |
Suggested primer 2: | AGGTCCACCTTCACCTCCTT |