| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150602 |
| Chromosome: | chromosome 1 |
| Location: | 4050863 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026400 | EXS1 | (1 of 1) PF03124 - EXS family (EXS); ERD1 homolog | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCAAACCCAACGCGGCCGGCACAAGTC |
| Internal bar code: | CCCCTGTCACGTCAGGCGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 99.3711 |
| LEAP-Seq n confirming: | 790 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAATAACAGCATTCAGA |
| Suggested primer 2: | AGGTCCGACACGTGGTACTC |