| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150621 |
| Chromosome: | chromosome 10 |
| Location: | 1729593 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACTATATCATCGAGCGCAAAGTTACTGA |
| Internal bar code: | GACGATCGAGGGGGCACGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.3598 |
| LEAP-Seq n confirming: | 776 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGAGCAGCAGAAGGATT |
| Suggested primer 2: | ATCACACATCACACGCCCTA |