| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150624 |
| Chromosome: | chromosome 12 |
| Location: | 41365 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483550 | VPE1 | (1 of 1) K01369 - legumain (LGMN); Vacuolar processing enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGCATGTCGCAGCGGCGCCAGGTCGG |
| Internal bar code: | GGGTAGGGCACGAAGGGTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.8457 |
| LEAP-Seq n confirming: | 647 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGACCCCTACACGGTAT |
| Suggested primer 2: | CCGCACACATTGTCACCTAC |