| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150660 |
| Chromosome: | chromosome 2 |
| Location: | 2228903 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089950 | IFT20 | (1 of 1) K16473 - intraflagellar transport protein 20 (IFT20); Intraflagellar transport protein 20 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCAGACTCGTCTTCTTGCCAAGTTCTT |
| Internal bar code: | TGGGAACCGTGGTGGCAACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 64 |
| LEAP-Seq percent confirming: | 99.3151 |
| LEAP-Seq n confirming: | 145 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAGGATGCGGATGACAAG |
| Suggested primer 2: | CATGTTTACAGCGAGGAGCA |