Insertion junction: LMJ.RY0402.150665_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.150665
Chromosome:chromosome 9
Location:4800460
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g397327 (1 of 1) PTHR11139:SF68 - DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTTGCGGCGCGTGGACGATCGCCACAC
Internal bar code:TCAACGCCTCGCGCGACAGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:199
LEAP-Seq percent confirming:55.1346
LEAP-Seq n confirming:1208
LEAP-Seq n nonconfirming:983
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCCCAAACTAAACCAAA
Suggested primer 2:GGTTGACACACACAGATGGC