| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150672 |
| Chromosome: | chromosome 6 |
| Location: | 1546934 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260050 | (1 of 27) IPR015947 - PUA-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTTGTGGAGGTGGGTGGAAGCCAGGCA |
| Internal bar code: | ACCGGTATTGTATTATTGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 83.1818 |
| LEAP-Seq n confirming: | 915 |
| LEAP-Seq n nonconfirming: | 185 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATTTGGTCGAGGAGCTTT |
| Suggested primer 2: | GGCATCGACTGAGGAGTAGC |