| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150672 |
| Chromosome: | chromosome 6 |
| Location: | 1547001 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260050 | (1 of 27) IPR015947 - PUA-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCATACGACAACCGCAGCGCGCGCCGCT |
| Internal bar code: | GCTCGGGCCAGTATGATTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 83 |
| LEAP-Seq percent confirming: | 99.802 |
| LEAP-Seq n confirming: | 504 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATTTGGTCGAGGAGCTTT |
| Suggested primer 2: | GGCATCGACTGAGGAGTAGC |