| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150674 |
| Chromosome: | chromosome 3 |
| Location: | 8448422 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199759 | GEX5,ORP3A | (1 of 1) PTHR10972:SF96 - OXYSTEROL-BINDING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGGCTCACTTGTCGCAGCCTATGCATG |
| Internal bar code: | AGAGGGGGGAGCATGATGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 99.4781 |
| LEAP-Seq n confirming: | 1525 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTAGACCGACCCGACAA |
| Suggested primer 2: | TCCATCATCACGATAGGCAA |