Insertion junction: LMJ.RY0402.150685_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.150685
Chromosome:chromosome 6
Location:8144877
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g305050 EEP5,CGL45 expressed protein Conserved in the Green Lineage; (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGTTATTCCTGATATATTGTATAACTAG
Internal bar code:TGGGGTGTAGTTCAAACCCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:873
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:428
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCTCGAAATGAAGCTGGG
Suggested primer 2:TGTTGATGTGTACCCCGCTA