| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150698 |
| Chromosome: | chromosome 3 |
| Location: | 1026888 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148450 | TEH1 | Acetyl-CoA thioesterase/hydrolase; (1 of 1) 3.1.2.1 - Acetyl-CoA hydrolase / Acetyl-CoA deacylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCCTTCAAACGCATGGGCTTTTCCAT |
| Internal bar code: | CACATGACCGGCGTCGCTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 99.7729 |
| LEAP-Seq n confirming: | 3075 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTAGCTTCGGGACTTTC |
| Suggested primer 2: | ATTACGATACGTTGAGCCCG |