Insertion junction: LMJ.RY0402.150706_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.150706
Chromosome:chromosome 7
Location:3920136
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g339104 (1 of 2) 3.6.3.19 - Maltose-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGCTCTACTACACTCGATCACGCACCG
Internal bar code:CTAAGTTTGCGAGTTCGGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:597
LEAP-Seq percent confirming:90.2174
LEAP-Seq n confirming:83
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCTGCTCACGAGACAAA
Suggested primer 2:TGTCCTACAGGACTGTCCCC