| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150715 |
| Chromosome: | chromosome 1 |
| Location: | 6980520 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050451 | TTL6 | (1 of 1) 1.2.1.24//6.3.2.25 - Succinate-semialdehyde dehydrogenase (NAD(+)) // Tubulin--tyrosine ligase / TTL; predicted tubulin tyrosine ligase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCCCGGAACTGAGGTTGTTCTGTTGTC |
| Internal bar code: | AGAGTTAAGCTTAACGCCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 66.4 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAAGTCTCGCTCGATCAC |
| Suggested primer 2: | AAGGTCCGGTAACACATTGC |