Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.150715 |
Chromosome: | chromosome_1 |
Location: | 6980568 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g050451 | TTL6 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGAATTGATGAATATCATACTATCGCACGT |
Internal bar code: | TGTGTTGGATTTTAAGTAAAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 845 |
LEAP-Seq percent confirming: | 92.4638 |
LEAP-Seq n confirming: | 1276 |
LEAP-Seq n nonconfirming: | 104 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGAAAGTCTCGCTCGATCAC |
Suggested primer 2: | AAGGTCCGGTAACACATTGC |