| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150720 |
| Chromosome: | chromosome 3 |
| Location: | 3741435 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169750 | FAP371 | Flagellar Associated Protein 371 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCAGGCTGTGTTCACTTAATCTTGCCA |
| Internal bar code: | CACCAATTTCCGCTTCGGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 95.9496 |
| LEAP-Seq n confirming: | 1066 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTCGTGAAAGTGGCAAG |
| Suggested primer 2: | GGGCTTGCAGTAAGTGCTTC |