Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.150720 |
Chromosome: | chromosome_3 |
Location: | 3741435 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g169750 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAAGCAGGCTGTGTTCACTTAATCTTGCCA |
Internal bar code: | CACCAATTTCCGCTTCGGCGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 663 |
LEAP-Seq percent confirming: | 95.9496 |
LEAP-Seq n confirming: | 1066 |
LEAP-Seq n nonconfirming: | 45 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGAGTCGTGAAAGTGGCAAG |
Suggested primer 2: | GGGCTTGCAGTAAGTGCTTC |