| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150760 |
| Chromosome: | chromosome 14 |
| Location: | 3365178 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630150 | (1 of 1) PTHR10131//PTHR10131:SF52 - TNF RECEPTOR ASSOCIATED FACTOR // TNF RECEPTOR-ASSOCIATED FACTOR FAMILY PROTEIN DDB_G0268444-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGACGGAGATGATGAAGATGAGGATGA |
| Internal bar code: | GGCTTTGGAAACCCGGACAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 95.8974 |
| LEAP-Seq n confirming: | 374 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTAATCCCCAACAATCCT |
| Suggested primer 2: | AGTGGCTGTAGCAGCAGGAG |