Insertion junction: LMJ.RY0402.150791_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.150791
Chromosome:chromosome 5
Location:1632834
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g243150 Carbonic anhydrase-related protein; (1 of 1) PF04357 - Family of unknown function (DUF490) (DUF490) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTTACTACAAGTATCAGCCCTATGCGCT
Internal bar code:GCCGTGGAGTCTTGTCTTCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:59
LEAP-Seq percent confirming:96.8254
LEAP-Seq n confirming:122
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTACTTTCGGTGTGGGT
Suggested primer 2:TCTGCAACAAACTACAGGCG