Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.150791 |
Chromosome: | chromosome 5 |
Location: | 1632834 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g243150 | Carbonic anhydrase-related protein; (1 of 1) PF04357 - Family of unknown function (DUF490) (DUF490) | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTACTACAAGTATCAGCCCTATGCGCT |
Internal bar code: | GCCGTGGAGTCTTGTCTTCGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 59 |
LEAP-Seq percent confirming: | 96.8254 |
LEAP-Seq n confirming: | 122 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGTACTTTCGGTGTGGGT |
Suggested primer 2: | TCTGCAACAAACTACAGGCG |