| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150798 |
| Chromosome: | chromosome 12 |
| Location: | 9286968 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541211 | putative protein kinase; (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGCCCTCAAGCCCGTCGCGCCATGAGT |
| Internal bar code: | CCCGGAGCGGGCACGCTGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.7646 |
| LEAP-Seq n confirming: | 2543 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTTGAAAAGAGAACGGC |
| Suggested primer 2: | GATTCTCGTGACGCAACTCA |