| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150808 |
| Chromosome: | chromosome_7 |
| Location: | 2921058 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g332650 | NCT1 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGTGTGAGGCTGTGTGCGGGGGCCGGCAGC |
| Internal bar code: | CCTTTCACAAACTGGGAAGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 77.4846 |
| LEAP-Seq n confirming: | 4639 |
| LEAP-Seq n nonconfirming: | 1348 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTTCCCAGTGGACCAGA |
| Suggested primer 2: | CTTCTTTCCCCTCCCATCTC |