| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150833 |
| Chromosome: | chromosome_1 |
| Location: | 2649750 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g015500 | CGL91 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGGAAGCGTACTTACACGATTTTGCACGC |
| Internal bar code: | AGTCATGATCATGCTAAGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 99.5801 |
| LEAP-Seq n confirming: | 1897 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTTGTGTCCACTCACTT |
| Suggested primer 2: | CGGGATGAGTGGTGAAGACT |