| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150845 |
| Chromosome: | chromosome 4 |
| Location: | 2959962 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224826 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATCTCGCGCACTCAACGCTCTTTATTA |
| Internal bar code: | GTCGACCGTTGCTTCGGCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 99.6536 |
| LEAP-Seq n confirming: | 863 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGAAGAAGTCCTTGCC |
| Suggested primer 2: | AACATGCGTATGCGGTATCA |