Insertion junction: LMJ.RY0402.150851_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.150851
Chromosome:chromosome 4
Location:2396926
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g221200 CGL109 Conserved in the Green Lineage; (1 of 1) IPR001005//IPR001965//IPR009057//IPR011011//IPR013083//IPR017877 - SANT/Myb domain // Zinc finger, PHD-type // Homeodomain-like // Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type // Myb-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGGGTGAAGAGCAAAAGGTCCGGACTCA
Internal bar code:CCTAGGTTTGCGGTCCGTGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:450
LEAP-Seq percent confirming:99.8178
LEAP-Seq n confirming:1096
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCGTCTGAGCGTAGTCCT
Suggested primer 2:TACATCCCATAACCCCCTGA