Insertion junction: LMJ.RY0402.150862_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.150862
Chromosome:chromosome 1
Location:4563158
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g031100 MET1,TEF30 Thylakoid enriched fraction, 30-kDa protein; (1 of 3) PTHR17130//PTHR17130:SF24 - MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25 // GAN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTACCCGCTGACGCCCCTCAGCTCAATGG
Internal bar code:GCTCGTTACGTTGAATCCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:807
LEAP-Seq percent confirming:99.6226
LEAP-Seq n confirming:1320
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGATGCAATAAAATGATGCG
Suggested primer 2:GCTGCCCTGTCCTTCAGTAG