| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150889 |
| Chromosome: | chromosome 13 |
| Location: | 3184995 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGTCCTGGTCCCGTACGGTATGCATGT |
| Internal bar code: | CTTTGTTTGGAAGAATGCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 70.6977 |
| LEAP-Seq n confirming: | 152 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCGCTTATGCAACGTAA |
| Suggested primer 2: | GGATGGCTGGACGAGTCTTA |