| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150897 |
| Chromosome: | chromosome_13 |
| Location: | 3741338 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g589450 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAAGCAAAACACAACTCGCTTTCACTCCA |
| Internal bar code: | TCGAAAACTGAGGACGTCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 97.0103 |
| LEAP-Seq n confirming: | 941 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATGCCTGGTTGTAAGCC |
| Suggested primer 2: | GAGGTAGCTGAACTCCACCG |