| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150897 |
| Chromosome: | chromosome 16 |
| Location: | 32854 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g696000 | (1 of 2) 2.7.7.23 - UDP-N-acetylglucosamine diphosphorylase / UDP-N-acetylglucosamine pyrophosphorylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGGACTTAGGTTCAATACGCGGACCAA |
| Internal bar code: | ACGTCATGCCACACCGGCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 99.8408 |
| LEAP-Seq n confirming: | 1254 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCGCCCACTAAGTACAA |
| Suggested primer 2: | CGGCGTATGTTTTGGAGTTT |