| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150900 |
| Chromosome: | chromosome 12 |
| Location: | 2235732 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508100 | (1 of 2) PTHR21580//PTHR21580:SF21 - SHIPPO-1-RELATED // O(6)-METHYLGUANINE-INDUCED APOPTOSIS 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGGAGTAAGGAACACTGAACACGTCGT |
| Internal bar code: | GTAGGTCTAAAGGCTACAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1069 |
| LEAP-Seq percent confirming: | 99.1379 |
| LEAP-Seq n confirming: | 115 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAAGAGAGAGGTTTGGC |
| Suggested primer 2: | GACAGGGACAGGAACAGGAA |