Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.150920 |
Chromosome: | chromosome_1 |
Location: | 5360964 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g037600 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCAGATGGCACAGCCCTCACCACTGTCCC |
Internal bar code: | GGGTGTCTAACTCTGGCGGGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 343 |
LEAP-Seq percent confirming: | 70.922 |
LEAP-Seq n confirming: | 200 |
LEAP-Seq n nonconfirming: | 82 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCATCCACCTAGGCACTC |
Suggested primer 2: | AAAATGTCAAACGGGTCAGC |