| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.150954 |
| Chromosome: | chromosome 2 |
| Location: | 1576041 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084950 | GUK1 | Guanylate kinase; (1 of 1) K00942 - guanylate kinase (E2.7.4.8, gmk) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCTGTCATGGTCAAGCTATGGTACAAA |
| Internal bar code: | TTCTACCGTGTTGCTCTGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 99.7778 |
| LEAP-Seq n confirming: | 449 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATGAGAGTGGCCGTGAT |
| Suggested primer 2: | GGCGTTCTTAGGAGCATCAG |