Insertion junction: LMJ.RY0402.150956_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.150956
Chromosome:chromosome 16
Location:5182297
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g683100 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGACACACTTGTTCGTATGCCCGTATG
Internal bar code:GGCAATATTAGCGTCGATGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:521
LEAP-Seq percent confirming:37.4806
LEAP-Seq n confirming:241
LEAP-Seq n nonconfirming:402
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTTGATACCTGGGTGCAT
Suggested primer 2:AACCCAGTGGCGTATGAGAC