| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150957 |
| Chromosome: | chromosome 10 |
| Location: | 5191749 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456800 | (1 of 2) PF09733 - VEFS-Box of polycomb protein (VEFS-Box) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCACACCTGCAACAGAGTCTACCTGTA |
| Internal bar code: | CGGTACTAATCGCGCACTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 99.4322 |
| LEAP-Seq n confirming: | 6129 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGGTGTTGTGCTGCTGGT |
| Suggested primer 2: | GACACTCGTGGGAGGTGATT |