| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.150960 |
| Chromosome: | chromosome 8 |
| Location: | 249010 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358553 | (1 of 1) IPR000104//IPR001810//IPR006553 - Antifreeze protein, type I // F-box domain // Leucine-rich repeat, cysteine-containing subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACCTCAAAAGTTCATACGCGCCAACTC |
| Internal bar code: | TAGTCGGGGTTGAGCCATGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1088 |
| LEAP-Seq percent confirming: | 98.7924 |
| LEAP-Seq n confirming: | 5154 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATGTGGGTACTGGTGGT |
| Suggested primer 2: | ACCGGTATTGTTTCTGCTCG |