Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.150960 |
Chromosome: | chromosome 8 |
Location: | 249014 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g358553 | (1 of 1) IPR000104//IPR001810//IPR006553 - Antifreeze protein, type I // F-box domain // Leucine-rich repeat, cysteine-containing subtype | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCACTGTGGGGATGACGCGGACGCGG |
Internal bar code: | ACAGCAGAGAGAGAGGGGCCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 493 |
LEAP-Seq percent confirming: | 99.6024 |
LEAP-Seq n confirming: | 1002 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCATGTGGGTACTGGTGGT |
Suggested primer 2: | ACCGGTATTGTTTCTGCTCG |