| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.150982 |
| Chromosome: | chromosome 12 |
| Location: | 239493 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g485250 | CSV5 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGATGTTATGACGCTGAGATTGTTGA |
| Internal bar code: | GGTTAACCGGGCTCAACCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 776 |
| LEAP-Seq percent confirming: | 99.5599 |
| LEAP-Seq n confirming: | 2262 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATGTAGGCGTCCCTTTG |
| Suggested primer 2: | AGACAGGATCAACCGACCAC |