| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.150984 |
| Chromosome: | chromosome 9 |
| Location: | 3923405 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392282 | DHC2 | Axonemal Dynein Heavy Chain 2; (1 of 1) PTHR10676:SF137 - DYNEIN HEAVY CHAIN 1, AXONEMAL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTTGGAAGGTGTCCAGCTTGTCCTCCCA |
| Internal bar code: | ATGTGTAAAAGAGAATATAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 97.8495 |
| LEAP-Seq n confirming: | 1001 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAAGGGAAGCTAGCGCAT |
| Suggested primer 2: | CACCTTGAACGGAATCACCT |