Insertion junction: LMJ.RY0402.151033_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.151033
Chromosome:chromosome 8
Location:2673362
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g373200 FXL7 FixL-like PAS domain protein; (1 of 11) PF13426 - PAS domain (PAS_9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGTATGCGCGATGAAACCCCTTGAAACA
Internal bar code:CACGAATGGTAGGGAAGGAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:170
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:456
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TACATGTTGCGCCTGATGAT
Suggested primer 2:GCGCCGACACTAGTAACACA