| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.151043 |
| Chromosome: | chromosome 14 |
| Location: | 2602288 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625850 | MMP32 | Matrix metalloproteinase; (1 of 1) IPR003980//IPR008752 - Histamine H3 receptor // Peptidase M11, gametolysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGATGTCGGCCCTTCCTTCGCCCGCCCT |
| Internal bar code: | GATATGCGTTATTCAGCGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 218 |
| LEAP-Seq percent confirming: | 99.734 |
| LEAP-Seq n confirming: | 6373 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTACCCGGTGAGAATCAG |
| Suggested primer 2: | ACACACACACACACACCACG |