| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151052 |
| Chromosome: | chromosome 7 |
| Location: | 164398 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313250 | (1 of 1) 3.6.3.17//3.6.3.25 - Monosaccharide-transporting ATPase // Sulfate-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGTACTCGGCGTCGTTGTAGCCGGGCA |
| Internal bar code: | AGAACAAGAATTTGAACCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 99.7858 |
| LEAP-Seq n confirming: | 3261 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAAGGGAGTGTGTTGGA |
| Suggested primer 2: | TGATCAGCAGACTGTGGGAG |