Insertion junction: LMJ.RY0402.151065_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.151065
Chromosome:chromosome 3
Location:8208908
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g199950 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAATGGGGTGGTGCGGGGTGCAGCGCTT
Internal bar code:CCAGCGCTATCCCGTTGGAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:89.8345
LEAP-Seq n confirming:380
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGCTTCTTGCTGAAGTC
Suggested primer 2:CTGCTTGCACAGGACCAGTA