| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151077 |
| Chromosome: | chromosome 12 |
| Location: | 697090 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494800 | DII1,IDA-IC28,IDA4,p28IDA4 | (1 of 1) K10410 - dynein light intermediate chain, axonemal (DNALI); Flagellar inner arm dynein light chain p28 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATCCAAACGCACATAAGACGTTGTAGAC |
| Internal bar code: | TATTCTAAAGTAGGGGGGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 99.7616 |
| LEAP-Seq n confirming: | 837 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTCGACGTGAGTGTTCC |
| Suggested primer 2: | AGGTTGATAACATCCAGGCG |