| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151111 |
| Chromosome: | chromosome 14 |
| Location: | 149966 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g608700 | ADT1 | (1 of 1) 2.6.1.39 - 2-aminoadipate transaminase / 2-aminoadipate aminotransferase; Putative 2-aminoadipate/L-kynurenine transaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGCAGCTGGGGGCGTGGGAAGTGCGAT |
| Internal bar code: | GGTACGTCGGACTCGGCAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCTTACGCCTTCACTTC |
| Suggested primer 2: | TCAACGTGTGCCTAGTCTGC |